Name | Sudan Black B |
Synonyms | CI 26150 C.I. 26150 Sudanblack Fat Black HB Fast Black HB Sudan Black B Solvent Black 2 Solvent Black 3 SUDAN BLACK B (CERT) C.I. Solvent Black 3 LABOTEST-BB LT00451850 fat black for microscopy C.I. Solvent Black 3 (8CI) sudan black B practical grade Sudan black b, high purity biological stain 1H-Perimidine, 2,3-dihydro-2,2-dimethyl-6-((4-(phenylazo)-1-naphthyl)azo)- 2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)naphthalen-1-yl]azo]-1H-perimidine 2,2-Dimethyl-6-{[4-(phenyldiazenyl)-1-naphthyl]-diazenyl}-2,3-dihydro-1H-perimidine 2,2-dimethyl-6-[(E)-{4-[(E)-phenyldiazenyl]naphthalen-1-yl}diazenyl]-2,3-dihydro-1H-perimidine |
CAS | 4197-25-5 |
EINECS | 224-087-1 |
InChI | InChI=1/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3 |
Molecular Formula | C29H24N6 |
Molar Mass | 456.54 |
Density | 1.4899 (rough estimate) |
Melting Point | 120-124°C(lit.) |
Boling Point | 552.68°C (rough estimate) |
Water Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. |
Solubility | Soluble in acetone and toluene, slightly soluble in ethanol, almost insoluble in water |
Appearance | Dark brown to dark brown and black powder |
Color | Very dark brown to black |
Maximum wavelength(λmax) | ['598 nm, 415 nm'] |
Merck | 13,8970 |
BRN | 723248 |
pKa | 2.94±0.40(Predicted) |
Storage Condition | Store at RT. |
Stability | Light Sensitive |
Refractive Index | 1.4570 (estimate) |
MDL | MFCD00006919 |
Physical and Chemical Properties | Black powder. Soluble in ethanol, toluene, acetone and other solvents. In concentrated sulfuric acid, it was purplish black, and after dilution, it was dark green blue, resulting in a blue to black precipitate. The addition of concentrated hydrochloric acid to the ethanol solution of the dye is blue black; The addition of concentrated sodium hydroxide solution is dark blue. |
Use | Biological stain, for bacterial and fat staining, used in histochemistry to distinguish paraffin and animal fat, myelin staining, white blood cell particles and Golgi apparatus staining, and lipid-like staining in cells and tissues. |
Risk Codes | R11 - Highly Flammable R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S24/25 - Avoid contact with skin and eyes. S16 - Keep away from sources of ignition. S37/39 - Wear suitable gloves and eye/face protection S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
WGK Germany | 3 |
RTECS | SD4431500 |
TSCA | Yes |
HS Code | 32041900 |
Hazard Class | IRRITANT |
Toxicity | LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 |
color index | 26150 |
biological field application | Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; Drug screening |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
introduction | Sudan black B is black powder and is soluble in solvents such as ethanol, toluene, acetone, etc. It is purple black in concentrated sulfuric acid, dark green blue after dilution, and produces blue to black precipitate. Adding concentrated hydrochloric acid to the ethanol solution of the dye is blue black; adding concentrated sodium hydroxide solution is dark blue. It is mainly used as a biological stain. |
application | Sudan black B is a diazo fat stain, used to dye neutral lipid frozen sections and paraffin sections of some lipoproteins. Under normal circumstances, it is dark brown or black powder. Sudan black B is one of the Sudanese dyes, which can be used to extract fingerprints and stain myeloid cells. |
use | biological stain, for bacterial and fat staining, used to distinguish paraffin and animal fat in histochemistry, myelin staining, white blood cell particles and Golgi apparatus staining, Lipid-like staining in cells and tissues. |
Production method | Using aniline, 1-naphthylamine, 2, 3-dihydro-2, 2-dimethyl [oral white] pyridine as raw materials, firstly diazotization of aniline, coupling with 1-naphthylamine, and then diazotization of the coupling product, and 2, 3-dihydro-2, 2-Dimethyl [oral white] pyridine coupling product. The finished product is filtered and dried.. |